UTILIZATION OF THE METHANOL MACERATIVES OF PRE-PUPAL STAGES OF THE BLACK SOLDIER FLY, Hermetia illucens L. (DIPTERA: STRATIOMYIDAE) FOR INHIBITION OF BACTERIAL GROWTH

  • VITTHALRAO B. KHYADE Sericulture Unit, Malegaon Sheti Farm, Agricultural Development Trust Baramati, Shardanagar, (Malegaon Khurd) Post Box No - 35, Baramati, Pune 413 115, Maharashtra, India. and Department of Zoology, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar Tal. Baramati Dist, Pune – 413115, India and Dr. APIS”, Shrikrupa Residence, Teachers Society, Malegaon Colony (Baramati), Dist. Pune – 413115, India.
Keywords: Antimicrobial Proteins (AMPs), Hermetia.illucens L, Salmonella typhimurium L, Escherichia coli L. and Pseudomonas aureginosa L.

Abstract

The attempt of the studies was carried with the aim of determination of the inhibition of the growth of three bacterial species (Salmonella typhimurium L., Escherichia coliL. and Pseudomonas aureginosa L.) through the use of whole-body extractives of pre-pupal stages of the black-soldier-fly, Hermetia.illucens L. (Order: Diptera; Family: Stratiomyidae). The whole-body extractives of pre-pupal stages of the black-soldier-fly, Hermetia.illucens L. (Order: Diptera; Family: Stratiomyidae) was prepared through the method of maceration. Completely Randomized Design (CRD) was followed for the experimentation. The attempt of experiments was consisting of six-groups of the treatment with three replications each. The groups of the treatment in the attempt include six different concentrations of whole-body extractives of pre-pupal stages of the black-soldier-fly, Hermetia.illucens L. (Order: Diptera; Family: Stratiomyidae) (50 mg per Lit.; 100 mg per Lit; 150 mg per Lit.; 200 mg per Lit.; 250 mg per Lit. and 300 mg per Lit.).  The antibiotic compound: chloramphenicol (thirty micrograms per disc-paper) was served as a positive control. The dimethyl sulfoxide was served as negative control. The zone of inhibition of the growth of bacterial species, Salmonella typhimurium (L.) through the use of extractives of pre-pupal stages of the black-soldier-fly, Hermetia.illucens L. (Order: Diptera; Family: Stratiomyidae) with concentration of 50; 100; 150; 200; 250 and 300 ppm in the present was 7.28 mm; 7.91 mm; 9.13 mm; 10.49 mm; 11.73 mm and 12.03 mm respectively. The zone of inhibition of the growth of bacterial species, Salmonella typhimurium (L.) through the use of antibiotic compound: Chloramphenicol was 14.76 mm. The zone of inhibition of the growth of bacterial species, Escherichia coli (L.) through the use of extractives of pre-pupal stages of the black-soldier-fly, Hermetia.illucens L. (Family: Diptera; Family: Stratiomyidae) with concentration of 50; 100; 150; 200; 250 and 300 ppm in the present was 7.13 mm; 8.92 mm; 9.74 mm; 9.86 mm; 10.03 mm and 10.88 mm respectively. The zone of inhibition of the growth of bacterial species, Escherichia coli (L.) through the use of antibiotic compound: Chloramphenicol was 27.64 mm. The zone of inhibition of the growth of bacterial species, Pseudomonas aeruginosa (L.) through the use of extractives of pre-pupal stages of the black-soldier-fly, Hermetia.illucens L. (Order: Diptera; Family: Stratiomyidae) with concentration of 50; 100; 150; 200; 250 and 300 ppm in the present was 7.18 mm; 9.96 mm; 11.47 mm; 12.56 mm; 13.44 mm and 14.69 mm respectively. The zone of inhibition of the growth of bacterial species, Pseudomonas aeruginosa (L.) through the use of antibiotic compound: Chloramphenicol was 21.34 mm. The zone of inhibition of growth of the bacterial species was found increasing significantly (P<0.05) according to the concentration of extractives of pre-pupal stages of the black-soldier-fly, Hermetia illucens L. (Order: Diptera; Family: Stratiomyidae). The concentration of 300 ppm of extractives of pre-pupal stages of the black-soldier-fly, Hermetia.illucens L. (Order: Diptera; Family: Stratiomyidae) was found effective for the inhibition of growth of bacterial species in the attempt.

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Published
2021-07-13
How to Cite
KHYADE, V. B. (2021). UTILIZATION OF THE METHANOL MACERATIVES OF PRE-PUPAL STAGES OF THE BLACK SOLDIER FLY, Hermetia illucens L. (DIPTERA: STRATIOMYIDAE) FOR INHIBITION OF BACTERIAL GROWTH. UTTAR PRADESH JOURNAL OF ZOOLOGY, 42(14), 109-118. Retrieved from https://mbiimph.com/index.php/UPJOZ/article/view/2280
Section
Original Research Article